antibody mouse anti-proliferating cell nuclear antigen (pcna)  (Santa Cruz Biotechnology)

Journal: Biomedical Reports

Article Title: TiO 2 NPs improve ultrasound response: CS/β-GP/TiO 2 NP hydrogel enabling on-demand administration

doi: 10.3892/br.2025.2020

Figure Lengend Snippet: Western blotting results of different composite hydrogels. The results of western blotting assay revealed no significant differences in cell protein expression among the different groups (P>0.05). PCNA, proliferating cell nuclear antigen; CS/β-GP, chitosan/β-glycerophosphate; TiO 2 NPs, titanium dioxide nanoparticles.

Article Snippet: The following materials were used: i) CS powder (95% deacetylation degree; cat. no. C105799; Aladdin Scientific Corp.), ii) β-GP pentahydrate (cat. no. D106347; Aladdin Scientific Corp.), iii) sodium fluorescein (NaF; cat. no. F105615; Aladdin), iv) TiO 2 NPs (20-40 nm; cat. no. NM000800; Beijing Solarbio Science & Technology Co., Ltd.), v) L929 murine fibroblast cells (cat. no. KGG1306-1), vi) RPMI-1640 medium (containing newborn calf serum, double antibiotics; cat. no. KGL1509-500), vii) Cell Counting Kit-8 (CCK-8) cell proliferation assay kit (cat. no. KGA9305-500), viii) LIVE/DEAD cell viability assay kit (cat. no. KGA9501-1000), ix) bovine serum albumin (BSA) (standard grade, heat-treated) (cat. no. KGL2314-10), x) bicinchoninic acid (BCA) protein quantification assay kit (cat. no. KGB2101-250), all from Jiangsu KeyGen Biotech Co., Ltd., xi) mouse anti-proliferating cell nuclear antigen (PCNA) antibody (1:1,000; cat. no. BM0104) and xii) goat anti-mouse IgG/HRP antibody (1:10,000; cat. no. BA1056) both from Boster Biological Technology Co. Ltd.

Techniques: Western Blot, Expressing, Titanium Dioxide

Journal: PLOS One

Article Title: Cysteine dioxygenase knockout and taurine deficiency impair mouse uterine adenogenesis by inhibiting epithelial cell proliferation and enhancing apoptosis

doi: 10.1371/journal.pone.0329503

Figure Lengend Snippet: (a-d) 10 days taurine supplementation to the Cdo KO mice (KO+Tau) increases the taurine level (a), the number of uterine glands marked by Foxa2 (b, c) and Foxa2 mRNA levels (d) in the uterine tissue. (e) Western Blot detection and analysis of PCNA protein levels in the uteri of the WT, Cdo KO and KO + Tau mice (n ≥ 3). (f) Western blot detection of BAX/BCL2 protein levels (up) and the analysis of BAX/BCL2 ration (down) in the uteri of the WT, Cdo KO and KO + Tau mice. WT: wild type. KO: Cdo KO. PND: Postnatal day. Scale bar represents 20 μm. *P < 0.05, **P < 0.01, ***P < 0.001.

Article Snippet: The membrane was blocked with 5% (w/v) nonfat dry milk in 0.05 mol/L pH 7.4 Tris buffered saline (TBS) for 1 h and incubated with rabbit anti-CDO antibody (ab53436, abcam, Cambridge, UK; 1:2000), mouse anti- proliferating cell nuclear antigen (PCNA) antibody (60097–1-Ig, Proteintech Group, Inc., IL, USA; 1:2000), rabbit anti-BCL2-Associated X Protein (BAX) antibody ( T40051 , Abmart Shanghai Co.,Ltd., China, 1:2000), rabbit anti-BCL2-Associated X Protein (BAX) antibody (ab182858, abcam, Cambridge, UK; 1:2000), and internal control rabbit anti-Tubulin antibody (K006154P, Beijing Solarbio Science & Technology Co.,Ltd., China, 1:2000) overnight at 4°C.

Techniques: Western Blot

Journal: Journal of Pharmaceutical Analysis

Article Title: Tailoring a traditional Chinese medicine prescription for complex diseases: A novel multi-targets-directed gradient weighting strategy

doi: 10.1016/j.jpha.2025.101199

Figure Lengend Snippet: Nao Yi Kang decoction (NYKD) inhibits glial cell activation and proliferation. (A, B) Immunofluorescent staining shows that NYKD inhibits astrocyte activation (green) and decreases the number of proliferating astrocytes: representative images of glial fibrillary acidic protein (GFAP) and proliferating cell nuclear antigen (PCNA) double staining (A) and statistic diagram of the number of double positive cells of GFAP and PCNA. (C, D) Immunofluorescent staining shows that NYKD inhibits microglia activation (green) and decreases the number of proliferating microglia: representative images of ionized calcium-binding adaptor molecule 1 (Iba1) and PCNA double staining (C) and statistic diagram of the number of double positive cells of Iba1 and PCNA (D). (E, F) Immunofluorescent staining shows that NYKD decreased the number of M1 microglia: representative images of Iba1 and cluster of differentiation 86 (CD86) double staining (E) and statistic diagram of the number of double positive cells of Iba1 and CD86 (F). (G, H) Immunofluorescent staining shows that NYKD increases the number of M2 microglia: representative images of Iba1 and arginase 1 (Arg1) double staining (G) and statistic diagram of the number of double positive cells of Iba1 and Arg1 (H). Data are presented as mean ± standard deviation (SD) ( n = 5). ∗∗∗ P < 0.001 and ∗∗∗∗ P < 0.0001. ns: not significant. ICH: intracerebral hemorrhage; Medium: medium dose; DAPI: diamidino-phenyl-indole.

Article Snippet: The primary antibodies used were mouse anti-glial fibrillary acidic protein (GFAP) (1:1500, CSB-MA009369A0m; Cusabio Biotech Co., Ltd., Wuhan, China), mouse anti-proliferating cell nuclear antigen (PCNA) (1:1000, 2586; Cell Signaling Technology, Danvers, MA, USA), rabbit anti-ionized calcium-binding adaptor molecule 1 (Iba1) (1:500, 17198; Cell Signaling Technology), rabbit anti-PCNA (1:1000, 13110; Cell Signaling Technology), rabbit anti-cluster of differentiation 86 (CD86) (1:200, 19589; Cell Signaling Technology), and rabbit anti-arginase 1 (Arg1) (1:1000, 93668; Cell Signaling Technology).

Techniques: Activation Assay, Staining, Double Staining, Binding Assay, Standard Deviation

Journal: Oxidative Medicine and Cellular Longevity

Article Title: Apoptosis in the Mammary Gland of Virgin Rats Subchronically Fed With a Vitamin A Deficient Diet

doi: 10.1155/omcl/6334165

Figure Lengend Snippet: VAD and further refeeding with a VAS diet cause changes in cell proliferation in the mammary gland. (A) Immunohistochemistry for PCNA in the mammary gland. The black arrows indicate positive PCNA + cells. Magnification 100×. The scale bar represents 25 μm. (B) Quantification of the percentage of PCNA + cells from the immunohistochemistry images shown in (A). Data are shown as representative images or mean values ± SDs ( n = 10).

Article Snippet: The sections were incubated with the following primary antibodies: 8 h in a humidified chamber at 4°C with mouse monoclonal antihuman BCL-2 (clon BCL-2/100; Catalog. No. AM287-5M, BioGenex, San Ramón, CA, USA), 30 min in a humidified chamber at 20°C with rabbit polyclonal antihuman BAX protein (Catalog. No. AR347-5R, BioGenex, San Ramón, CA, USA), and for 4 h in a humidified chamber at 20°C with mouse monoclonal anti-rat proliferating cell nuclear antigen (PCNA) (clon PC10; Catalog. No. AM252-5M, BioGenex, San Ramón, CA, USA).

Techniques: Immunohistochemistry

Journal: Oxidative Medicine and Cellular Longevity

Article Title: Apoptosis in the Mammary Gland of Virgin Rats Subchronically Fed With a Vitamin A Deficient Diet

doi: 10.1155/omcl/6334165

Figure Lengend Snippet: VAS and further refeeding with a VAS diet cause changes in apoptosis and proliferation in the mammary gland. Determination of the TUNEL + /PCNA + cell ratios from the immunohistochemistry images shown in Figures A and A. The data are presented as the means ± SDs ( n = 4).

Article Snippet: The sections were incubated with the following primary antibodies: 8 h in a humidified chamber at 4°C with mouse monoclonal antihuman BCL-2 (clon BCL-2/100; Catalog. No. AM287-5M, BioGenex, San Ramón, CA, USA), 30 min in a humidified chamber at 20°C with rabbit polyclonal antihuman BAX protein (Catalog. No. AR347-5R, BioGenex, San Ramón, CA, USA), and for 4 h in a humidified chamber at 20°C with mouse monoclonal anti-rat proliferating cell nuclear antigen (PCNA) (clon PC10; Catalog. No. AM252-5M, BioGenex, San Ramón, CA, USA).

Techniques: TUNEL Assay, Immunohistochemistry